JAST
2012 March;3(1):121-127.
Published online 2012 February 13.
doi:http://dx.doi.org/10.5355/JAST.2012.121
|
| Copyright ⓒ 2010 Journal of Analytical Science & Technology |
| Analysis of Mercurial Preservatives in Different Vaccines by Cold Vapor Atomic Absorption Spectrophotometry |
| Pranvera Lazo1*, Merita Kucuku2 |
1 University of Tirana, Faculty Natural Sciences, Dept. of Chemistry, Tirana, Albania
2 National Center of Drugs Control, R. “R. Cerova” No. 2/1, Tirana, Albania |
|
Corresponding Author:
Pranvera Lazo ,Tel: +355-4-222-32-120, Email: pranveralazo@gmail.com |
 |
ABSTRACT |
 |
|
| Vaccines with low concentration of mercurial preservatives, such as thiomersal, are in use in Albania. A simple and a sensitive method by using CVAAS method for the determination of mercury in vaccine samples is described. This method is based on a digestion procedure that transforms organic mercury to Hg2+. Digestion is a critical step to destroy microorganism that could combine with Hg2+ and metabolize into ethyl mercury, thus preventing the mercury determination via CVAAS method. Negative chemical interferences were observed, after acid digestion of vaccine samples by obtaining low repeatability of the results.
The method was improved by using heating (80℃-90℃), UV radiation and digestion with strong oxidants and acids to transform organic mercury into Hg2+ for CVAAS determination. The accuracy of the analysis was checked by recovery of the spikes which was in the range of 98-102%. The method yielded reproducible results with RSD<5.5%.
|
| Keywords: vaccine, mercurial preservatives, UV radiation, thermal treatment, digestion, CVAAS |
|
|
 |
 Free archive |
|
Anyone may access any past or current articles without logging in.
|
| Register for e-submission |
|
Register here to access the e-submission system of JAST for authors and reviewers.
|
| Journal e-mail Alerts |
|
Don't forget to sign up for journal e-mail alerts so you can keep up to date with all the articles as they are published.
|
| Manuscript Submission |
|
To submit a manuscript, please visit the JAST e-submission management system at http://submit.jastmag.org, read the Instructions for Authors, and log into the JAST e-submission system. For assistance with manuscript submission, please contact: cheong@kbsi.re.kr.
|
|
 |
|
|
|